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Part:BBa_K2996505

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


Lure sequence and DBD binding site spaced by 15 base pairs

After testing the proper length of spacer in part BBa_K2996504,we knew that 15 base pairs is the best spacer.We mutated some base pairs by point mutation PCR,and made mutated target of NdCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 24
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 24
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


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